منابع مشابه
Ligand-directed gene targeting to mammalian cells by pseudotype baculoviruses.
The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) can infect a variety of mammalian cells, as well as insect cells, facilitating its use as a viral vector for gene delivery into mammalian cells. Glycoprotein gp64, a major component of the budded AcMNPV envelope, is involved in viral entry into cells by receptor-mediated endocytosis and subsequent membrane fusion. We ...
متن کاملManipulating mammalian genome by gene targeting.
The development of strategies which allow the inactivation of specific murine genes by homologous recombination in embryonic cells has revolutionized biological science in the last 10 years. A large number of mice carrying genetic lesions, generated by gene targeting technology, has tremendously increased our knowledge in many areas of biology, culminating in the identification of mouse models ...
متن کاملThe molecular basis of multiple vector insertion by gene targeting in mammalian cells.
Gene targeting using sequence insertion vectors generally results in integration of one copy of the targeting vector generating a tandem duplication of the cognate chromosomal region of homology. However, occasionally the target locus is found to contain >1 copy of the integrated vector. The mechanism by which the latter recombinants arise is not known. In the present study, we investigated the...
متن کاملMechanisms of double-strand-break repair during gene targeting in mammalian cells.
In the present study, the mechanism of double-strand-break (DSB) repair during gene targeting at the chromosomal immunoglobulin mu-locus in a murine hybridoma was examined. The gene-targeting assay utilized specially designed insertion vectors genetically marked in the region of homology to the chromosomal mu-locus by six diagnostic restriction enzyme site markers. The restriction enzyme marker...
متن کاملGene targeting in embryonic stem cells.
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1993
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.67.720